Sperm Bioassay

An alternative method to the mouse embryo bioassay is to use sperm motility as a method to detect potential embryo toxins. Hamster sperm bio-assay has been used to assay media and medium components for potential toxins (318-321). This assay utilizes both the number of motile sperm and degree of motility to determine the suitability of media to support embryo development. An advantage of this test is that it can be performed in four to six hours as opposed to the several days for the embryo bioassay. Unfortunately, however, there is little clinical data on the applicability of such tests.

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