The fertilized oocytes are further cultured up to day 2 or 3, and then embryo transfer is performed. Assisted hatching is performed to avoid reduced implantation due to a hardened zona pellucida. When a large number of embryos have been formed, alternative approaches could be either an extended culture to the blastocyst stage or a double transfer (62). A double transfer is performed on day 2 or 3 and a blastocyst transfer on day 5 or 6. The embryo transfer technique is the same as that employed for conventional IVF.
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