Inhibition of Dendritic Cell Generation and Function by Serum from Prostate Cancer Patients Correlation with Serum Free PSA

Maryam Aalamian-Matheis1, Gurkamal S. Chatta2, Michael R. Shurin3, Edith Huland4, Hartwig Huland4, and Galina V. Shurin5

department of Medicine, Johns Hopkins University, Baltimore, MD, USA

2Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA

3Department of Pathology and Immunology, University of Pittsburgh, Pittsburgh, PA, USA

^Department of Urology, University Clinics Hamburg-Eppendorf, Hamburg, Germany

'Department of Pathology, Pittsburgh, University of Pittsburgh, PA, USA, [email protected]

Abstract. Tumor produces a number of immunosuppressive factors that block maturation of dendritic cells (DCs). Here, we demonstrated that endogenous factors presented in the serum of patients with prostate cancer (CaP) inhibited the generation of functionally active DCs from CD14+ monocytes in vitro. We have shown a significant inhibitory potential of serum obtained from patients with CaP and benign prostate hyperplasia benign prostatic hyperplasia (BPH) when compared with serum from healthy volunteers. As assessed by flow cytometry, expression of CD83, CD86, and CD40 molecules was strongly inhibited by CaP and BPH serum. In addition, these DCs were weak stimulators of allogeneic T cell proliferation when compared with DCs produced in the presence of healthy volunteer serum. Statistical analysis of the results revealed a positive relationship between the inhibition of expression of DC markers CD83 and CD80 and the levels of serum-free prostate-specific antigen (PSA). These data suggest that the DC system may be impaired in CaP patients.

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