State of the

An ideal technology to directly characterize protein-ligand binding would have the following properties: (1) it would require no labeling of the target or small molecules with radioisotopes, fluorophores, or other moieties, and no covalent modification to immobilize the protein target or small molecules on a surface would be necessary; (2) the ideal technique could selectively identify the compound that is responsible for the observed output; (3) it would be solution-based, and amenable to all cofactors, salts, metal ions, and detergents necessary for proper protein folding and stability; and (4) it would require only modest amounts of a purified protein target for its implementation.

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