Reverse Screening with GPC Spin Columns

A proposed method for screening mixtures of potential drug candidates, using GPC spin columns, is to analyze by ESI-MS the retained compounds after elution of the protein from the spin column [36]. In such studies, the absence of a ligand indicates strong affinity towards a target while the presence of a ligand indicates much weaker or no affinity. The disadvantage of this reverse GPC spin column screening methodology is that all the retained compounds in a mixture have to be eluted from the GPC spin column, identified and quantitated, while in the direct GPC spin column methodology only the desired non-covalent binders are detected in the eluate.

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