Ratio of [Peptide [CaMnai

Fig. 11.7 Sharp-break PLIMSTEX curves at high protein concentration [23]. Line (a): melittin (a 26-amino-acid peptide) titration. Line (b): mastoparan (a 16-amino-acid peptide) titration of 15 ||M Ca2+-saturated porcine calmodulin (CaM-4Ca) in 50 mM HEPES, 100 mM KCl, 0.49 mM Ca2+, 99% D2O, apparent pH 7.4. Data points are based on the average of two runs for each titration system, and the breaking point clearly indicates 1:1 protein-ligand binding stochiometry.

accessible hydrophobic domains of CaM [62]). Melittin binding to Ca2+-saturated CaM (holo-CaM) appears to be a good test for PLIMSTEX.

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