Kinetic Study of Forward HD Exchange RasGDP with Different [Mg2B

To determine the appropriate H/D exchange time for the titration, we first determined the forward H/D exchange kinetics of ras-GDP (1.5 |M) as a function of [Mg2+]. We chose this protein concentration for the kinetics of H/D exchange at different [Mg2+] to [ras-GDP] ratios because we had a limited amount of protein and the initiation of H/D exchange by diluting the deuterated buffer would not cause significant dissociation of the ternary complex at this protein level [40]. Figure 11.3 shows the deuterium uptake versus the time of H/D exchange for three

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