Fig. 7.21 Kinetic MS binding study quantifying bound marker -dissociation experiment. Total binding (▼) of NO 711 (20 nM; incubation temperature 37 0C) to mGATl. GABA (10 mM) was added after 60 min preincubation to initiate dissociation (t = 0 min). Nonspecific binding (o) was determined as binding of NO 711 in the presence of 10 mM GABA. One representative example is shown. Data points represent each mean G SEM from triplicate values.
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