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Fig. 11.3 Forward H/D exchange kinetics for Ras-GDP with different [Mg2+]. Line (a), diamonds: [Mg2+]free/[Ras-GDP]total = 0. Line (b), triangles: [Mg2+]free/[Ras-GDP]total = 15. Line (c), circles: [Mg2+]free/ [Ras-GDP]total = 500. Conditions: 1.5 |mM Ras-GDP, in 50 mM HEPES/ 100 mM KCl, apparent pH 7.4, 90% D2O, T = 21.5 °C. Waters CapLC and a Q-TOF Ultima were used for LC/ESI-MS analysis.

Fig. 11.3 Forward H/D exchange kinetics for Ras-GDP with different [Mg2+]. Line (a), diamonds: [Mg2+]free/[Ras-GDP]total = 0. Line (b), triangles: [Mg2+]free/[Ras-GDP]total = 15. Line (c), circles: [Mg2+]free/ [Ras-GDP]total = 500. Conditions: 1.5 |mM Ras-GDP, in 50 mM HEPES/ 100 mM KCl, apparent pH 7.4, 90% D2O, T = 21.5 °C. Waters CapLC and a Q-TOF Ultima were used for LC/ESI-MS analysis.

Table 11.2 Average rate constants and number of amide hydrogens in each rate group for H/D exchange of ras-GDP under different Mg concentrations. Measurements were made in 100 mM KCl/50 mM HEPES, 90% D2O, apparent pH of 7.4, T = 21.5 + 0.5 °C. The final [ras-GDP]tot in H/D exchange media was 1.5 mM. EDTA was used to control [Mg2+] in solution.

Table 11.2 Average rate constants and number of amide hydrogens in each rate group for H/D exchange of ras-GDP under different Mg concentrations. Measurements were made in 100 mM KCl/50 mM HEPES, 90% D2O, apparent pH of 7.4, T = 21.5 + 0.5 °C. The final [ras-GDP]tot in H/D exchange media was 1.5 mM. EDTA was used to control [Mg2+] in solution.

Rate constants and number of amides [Mg2+]/[ras-GDP]tot

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