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Overview of H/D Exchange Technologies

Dramatic advances in mass spectrometry and improvements in the various steps within the experimental hydrogen exchange procedures have resulted in the development of automated systems for high-throughput, high-resolution H/D exchange analysis [6, 8, 40-44].

The system (Fig. 12.1), described in this section, incorporates the latest of these enhancements, including solid phase proteolysis, automated liquid handling and streamlined data reduction software [8].

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