H

Fig. 5.16 Demonstration of MS-based bioassay functionality. Injections represent standard solutions of 1 mM digoxin samples. MS instrument: Ion-trap mass spectrometer (LCQ Deca, Thermo Electron). m/z 651 represents the [(M-S)+H]+ ion of digoxin with S indicating one sugar moiety; m/z 798

represents the ammonium ion of digoxin. 1: Affinity protein and pH dissociation solution replaced by buffer solutions. 2: pH dissociation solution replaced by buffer solution. 3: All MS-based bioassay solutions installed.

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