Displacement Experiments of Known Binders

At this point in time, no GPC spin column ESI-MS experiments have reported the displacement of a prepared non-covalently bound ligand with a protein with an even stronger binder. (Related competition experiments were described above in Section 2.3.3.1.) This type of experiment could be easily implemented using the GPC spin column/ESI-MS methodology and was recently demonstrated in the related screening methodology, utilizing in tandem the GPC and reversed phase HPLC methods with ESI-MS detection [31] (see Chapter 3). Typical experiments that can be routinely implemented using GPC spin column/ESI-MS techniques include the displacement of storosporine by stronger binders to kinases and the non-displacement/displacement of storosporine by allosteric binders.

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