Disorderdepleted Mutant Preserved Ordered Structure

Four slightly differing deletion mutants of TM0160 devoid of the C-terminal disordered region were designed and prepared (Fig. 12.3a). It was important to determine if the truncated versions had the same folding patterns as the full-length protein in the regions with retained sequence. To answer the question, the exchange patterns of full-length TM0160 and its D3 deletion mutant were compared (Fig. 12.3b). Virtually identical exchange patterns of the two proteins in the retained regions indicated the preservation of native folded structure in the deletion mutant D3.

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