Since its introduction some 20 years ago, MALDI-MS has been established as a standard technique for a large variety of applications within the field of bioanalyt-ical mass spectrometry, ranging from protein identification to enzyme activity screening. Quantitative analysis has long been a challenge, but, with the use of isotopically labelled standards, it is steadily obtaining more attention.
In contrast to established MALDI-MS techniques, DIOS-MS is a comparatively new technique. However, over the last five years, it has been gaining steadily more attention and promising results have already been obtained in all those cases, where interference from classic MALDI matrices needed to be avoided. Owing to the fact that DIOS-MS is still a juvenile technique, it is hard to predict future developments, but especially in the field of silicon modifications further promising developments can be foreseen, accompanied by new applications.
SAMDI as a merger of the rapidly growing field of SAMs and the established MALDI-MS is an even more recent technique. Interesting assay formats and applications have already been described combining the selectivity of SAMs with the efficiency of MALDI. Nevertheless, there are, up to now, too few applications in order to predict in which way SAMDI is going to develop. For future applications, especially in the field of medicinal chemistry with its evident need for high-throughput systems, the parallel immobilization of various biomolecules to one SAM may turn out to be a versatile tool whenever rapid screening of drug candidates, enzymes or inhibitors is concerned.
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