Purification and Cloning of Interferon Alpha

'Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA 2PBL Biomedical Laboratories, 131 Ethel Road West, Suite 6, Piscataway, NJ 08854, USA [email protected]

1 The Interferons 23

2 Purification of Human Leukocyte Interferon 24

2.1 Production 26

2.2 High-Performance Liquid Chromatography for Protein Purification 26

2.3 Multiple Species of Leukocyte Interferon 30

2.4 Carbohydrate Content 30

3 Identification and Cloning of the Recombinant

Human IFN-a Species 31

4 The Recombinant Human IFN-a Genes and Proteins 33

5 Concluding Summary 33

6 References 34

Abstract Interferon alpha (IFN-a) was approved by the US Food and Drug Administration on June 5, 1986 and paved the way for development of many other cytokines and growth factors. Nevertheless, we have barely touched the surface of understanding the multitude of human IFNs. This paper reviews the history of the purification of human leukocyte IFN, the cloning of the IFN-as, and the current state of knowledge of human interferon alpha genes and proteins.

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