In Chapter 11, we described DNA sequencing and the polymerase chain reaction, two applications of DNA replication techniques. Here, we examine four additional techniques for manipulating DNA. One is the use of genetic recombination to create an inactive, or "knocked-out," gene. The second is the use of DNA chips to detect the presence of many different sequences simultaneously. The third is the use of antisense RNA and RNA interference to block the translation of specific mRNAs. The fourth is a method for determining which proteins interact in a cell.
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This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.