Possession Of Which Feature Is Not Desirable In A Vector For Gene Cloning

1. Restriction enzymes a. play no role in bacteria.

b. cleave DNA at highly specific recognition sequences.

c. are inserted into bacteria by bacteriophages.

d. are made only by eukaryotic cells.

e. add methyl groups to specific DNA sequences.

2. When fragments of DNA of different sizes are placed in an electrical field, a. the smaller pieces migrate most quickly to the positive pole.

b. the larger pieces migrate most quickly toward the positive pole.

c. the smaller pieces migrate most quickly toward the negative pole.

d. the larger pieces migrate most quickly toward the negative pole.

e. the smaller and larger pieces migrate at the same rate.

3. From the list below, select the sequence of steps for cloning a piece of foreign DNA into a plasmid vector, introducing the plasmid into bacteria, and verifying that the plasmid and the insert are present:

(1) Transform competent cells

(2) Select for the lack of antibiotic resistance gene #1 function

(3) Select for the plasmid antibiotic resistance gene #2 function

(4) Digest vector and foreign DNA with EcoRl, which inactivates antibiotic resistance gene #1

(5) Ligate the digested DNA together with the foreign DNA

a. 45132

b. 45123

c. 13425

d. 32145

e. 13254

4. Possession of which feature is not desirable in a vector for gene cloning?

a. An origin of DNA replication b. Genetic markers for the presence of the vector c. Multiple recognition sites for the restriction enzyme to be used d. One recognition site each for one to several different restriction enzymes e. Genes other than the target for cloning

5. RNA interference (RNAi) inhibits a. DNA replication.

b. RNA synthesis of specific genes.

c. recognition of the promoter by RNA polymerase.

d. transcription of all genes.

e. translation of specific mRNAs.

6. Complementary DNA (cDNA)

a. is produced from ribonucleoside triphosphates.

b. is produced by reverse transcription.

c. is the "other strand" of single-stranded DNA.

d. requires no template for its synthesis.

e. cannot be placed into a vector because it has the opposite base sequence of the vector DNA.

7. In a genomic library of frog DNA in E. coli bacteria, a. all bacterial cells have the same sequences of frog DNA.

b. all bacterial cells have different sequences of DNA.

c. each bacterial cell has a random fragment of frog DNA.

d. each bacterial cell has many fragments of frog DNA.

e. the frog DNA is transcribed into mRNA in the bacterial cells.

8. An expression vector requires all of the following, except a. genes for ribosomal RNA.

b. a selectable genetic marker.

c. a promoter of transcription.

d. an origin of DNA replication.

e. restriction enzyme recognition sites.

9. "Pharming" is a term that describes a. animals used in transgenic research.

b. plants making genetically altered foods.

c. synthesis of recombinant drugs by bacteria.

d. large-scale production of cloned animals.

e. synthesis of a drug by a transgenic animal in its milk.

10. In DNA fingerprinting, a. a positive identification can be made.

b. a gel blot is all that is required.

c. multiple restriction digests generate unique fragments.

d. the polymerase chain reaction amplifies finger DNA.

e. the variability of repeated sequences between two restriction sites is evaluated.

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Responses

  • orlando
    What are the desirable features of a cloning vector?
    7 years ago
  • alida
    What are the 3 desirable functions of a cloning vector?
    7 years ago

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