Recombinant DNA and Biotechnology

Of the many horrible legacies of the wars of the twentieth century, perhaps none is so lasting as the littering of the countryside with land mines. These inexpensive plastic shells filled with trinitrotoluene (TNT) are built to explode and injure whatever steps on the soil above them. Currently, the most common way of finding land mines is to poke around the soil with a stick—a precarious occupation at best. Since the mines are made of plastic, metal detectors do not work. The agricultural systems of many of the countries with large numbers of land mines, such as Cambodia and Angola, are based on manual labor, so where there are land mines, there cannot be a farm. Clearly, the world needs a sensitive, non-lethal land mine detector.

Enter biotechnology. Neal Stewart, at the University of North Carolina, is developing plants that can detect land mines in a field and show their locations remotely. His method is an excellent example of the application of knowledge of gene transcription and translation as well as of genetic engineering. In Chapter 14, we saw that the control of eukary-otic gene transcription lies at the promoter, a DNA sequence where RNA polymerase and other proteins bind to initiate transcription of the adjacent gene. Certain bacteria have a promoter that is sensitive to TNT, and the binding of a tiny amount of this chemical activates an adjacent gene.

Plants would be ideal biosensors for land mines, as seeds can be spread widely and evenly in a suspect field. But what gene could "announce" the presence of TNT by making a detectable protein? It turns out that certain jellyfish make a protein that fluoresces green when ultraviolet light ("black light") is shone on it. Stewart has introduced this gene into a plant, placing it alongside the TNT-sensitive promoter. When these plants are grown near a land mine, their roots take up TNT, and their leaves will glow in ultraviolet light. Of course, having people plant these seeds or shine a black light on these plants as they walk through the field would bring us back to the old, dangerous way of detecting land mines. To solve these problems, seeding and remote sensing could be done from airplanes or helicopters flying over the field.

This story—from problem to solution—has been repeated many times in the past two decades. The products of recombinant DNA technology range from life-saving drugs that there is no other way to make in adequate amounts to crop plants with improved agricultural characteristics. Although the basic

A Land Mine Detector Plants can be genetically engineered to express green fluorescent protein from a jellyfish gene. When this "glow-in-the-dark"gene is linked so that it is activated by the presence of TNT in the soil, such plants can act as biosensors to detect the presence of explosive land mines.

Landmine Detecting Plants Glowing

techniques of DNA manipulation have been called revolutionary, most of them come from the knowledge of DNA transcription and translation that we described in earlier chapters. The upshot of this technology is that today we can take a piece of DNA from any source, attach it chemically to any other DNA—making a recombined DNA molecule that has never existed before in the entire evolutionary history of life—and then use this unique DNA for whatever purpose and in whichever target organism we wish. This ability gives humans unprecedented power over life in general.

We begin this chapter with a description of how DNA molecules can be cut into smaller fragments and how fragments from different sources can be covalently linked to create recombinant DNA in a test tube. Recombinant (or any other) DNA can then be introduced into a suitable prokary-otic or eukaryotic host cell. Sometimes, the purpose of adding a new gene to a host cell or organism is to ask an experimental question about the role of that gene that can be answered by placing it in a new environment. In other instances, the purpose is to coax the host cell to make a new gene product.

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Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

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