O

Tuna

The number 1 indicates an invariant position in the cytochrome c molecule (i.e., all the organisms have the same amino acid in this position) and that the position is probably functionally very significant.

Side chains marked by red arrows interact with the heme group.

Tuna

Rice

Acidic side chains

Rice

Acidic side chains

D Aspartic acid [E Glutamic acid Basic side chains H Histidine K Lysine R Arginine

Hydrophobic side chains

[F Phenylalanine CO Isoleucine [L Leucine M Methionine

[V Valine [Y Tyrosine W Tryptophan A Alanine Other

Cysteine

Proline

Glutamine

Asparagine

Serine

Threonine

Glycine rn

Position in sequence

Number of amino acids at the position

Human, chimpanzee Rhesus monkey Horse Donkey Cow, pig, sheep Dog Rabbit Gray whale Gray kangaroo

Chicken, turkey Pigeon Pekin duck Snapping turtle Rattlesnake Bullfrog Tuna Dogfish

Samia cynthia (moth) Tobacco hornworm moth Screwworm fly Drosophila (fruit fly) Baker's yeast

Candida krusei (yeast) Neurospora crassa (mold) Wheat Sunflower Mung bean Rice Sesame

The number 1 indicates an invariant position in the cytochrome c molecule (i.e., all the organisms have the same amino acid in this position) and that the position is probably functionally very significant.

Side chains marked by red arrows interact with the heme group.

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