Method

Bright light source (energy for photosynthesis)

Algae were rapidly killed and their metabolites partially extracted by putting the cells in boiling ethanol.

The plant extract was ^ spotted here and run in two directions to separate compounds from one another.

First run

Algae were rapidly killed and their metabolites partially extracted by putting the cells in boiling ethanol.

The plant extract was ^ spotted here and run in two directions to separate compounds from one another.

Paper chromatogram -

First

Second run

After separation, the chromatogram was overlaid with X-ray film that the radiation "exposed." Each dark spot is a compound labeled with 14C.

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Essentials of Human Physiology

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