Experiment

Question: Which component of a bacteriophage—DNA or protein—is the hereditary material that enters a bacterial cell to direct the assembly of new virus particles?

Experiment 1

Experiment 2

1 a T2 phage are grown in a medium containing 32P (P is an element in DNA but not in proteins).

1b| T2 phage are grown in a medium containing 35S (S is an element in proteins but not in DNA).

32P-containing DNA

MEthOd 35S-containing protein coats

Bacteria

Bacteria

32P-containing DNA

The labeled viruses are used to infect bacteria.

After a short time, mixing in a blender detaches viruses from bacterial cells.

Centrifuging forces the bacterial cells to the bottom of the tube, forming a pellet. Supernatant fluid contains the viruses.

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