D

0 1 2 3 4 5 6 Total unlabeled hormone (concentration)

By repeating the process, adding different amounts of unlabeled hormone, it is possible to create a standard curve that shows the relationship between concentration of unlabeled hormone and the amount of labeled hormone that remains bound to antibodies.

0 1 2 3 4 5 6 Total unlabeled hormone (concentration)

42.15 An Immunoassay Measures Hormone Concentration

The sample to be measured is added to a mixture that replicates the conditions in step 1.The sample's effect on decreasing the amount of labeled, bound hormone is compared to the standard curve, thus revealing the concentration of unlabeled hormone in the sample of interest.

The dose that stimulates half the maximum response is a measure of sensitivity to the hormone.

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Essentials of Human Physiology

Essentials of Human Physiology

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