A DNA copy of mRNA can be made by reverse transcriptase

A much smaller DNA library—one that includes only the genes transcribed in a particular tissue—can be made from complementary DNA, or cDNA (Figure 16.8). Recall that most eukaryotic mRNAs have a poly A tail—a string of adenine residues at their 3' end (see Figure 14.9). The first step in cDNA production is to extract mRNA from a tissue and allow it to hybridize with a molecule called oligo dT, which consists of a string of thymine residues (the "d" indicates de-oxyribose). The oligo dT hybridizes with the poly A tail of the mRNA. The oligo dT serves as a primer, and the mRNA as a template, for the enzyme reverse transcriptase, which synthesizes DNA from RNA. In this way, a cDNA strand complementary to the mRNA is formed.

A collection of cDNAs from a particular tissue at a particular time in the life cycle of an organism is called a cDNA library. Messenger RNAs do not last long in the cytoplasm and are often present in small amounts, so a cDNA library is a "snapshot" that preserves the transcription pattern of the cell. cDNA libraries have been invaluable in comparisons of gene expression in different tissues at different stages of development. Their use has shown, for example, that up to one-third of all the genes of an animal are expressed only during prenatal development. Complementary DNA is also a good starting point for the cloning of eukaryotic genes. It is especially useful for cloning genes expressed at low levels in only a few cell types.

Was this article helpful?

0 0
Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

Get My Free Ebook

Post a comment