The purity of the effector cells is essential for the interpretation of the results. The methods to purify mononuclear phagocytes are discussed elsewhere in this volume (see Chapters 1 and 2). Primary cultures can easily be contaminated by few NK cells but sufficient to produce enough interferon to activate macrophages. This is a particular concern for human monocytes, where the starting leukocyte population has between 5-10% monocytes, and less of a problem for peritoneal macrophages that represent from 50-60% of the resident population to over 90% in the thioglycollate-induced exudate.
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