Preparation of nuclear ex

Equipment and reagents

• Sterile 60 mm tissue culture dishes

• Table-top centrifuge

• Microcentrifuge

• 25-gauge needles

• Macrophage population of choice (see Chapters 1 and 2}

• NE buffer; 10 mM Tris pH 7.4,10 mM NaCl, 6 mM MgCl2

• Phenylmethyl sulfonyl fluoride {PMSF, Sigma Chemical Co.)

• Aprotinin (Sigma Chemical Co.)

• Leupeptin (Sigma Chemical Co.)

Was this article helpful?

0 0

Post a comment