Macrophages have long been recognized as a critical component of innate and acquired immune responses. The recent explosion of interest in evolutionary, genetic, and biochemical aspects of cellular receptors responsible for microbe recognition has focused renewed scientific attention on macrophages, and has highlighted the need for an up-to-date summary of laboratory techniques effective for the isolation, identification, and functional analysis of these cells. The information in this book aims to provide investigators with a concise compilation of experimental techniques appropriate for studying diverse aspects of macrophage biology. Each chapter provides an overview of a relevant experimental topic as well as detailed and specific protocols for related laboratory procedures. I anticipate that this information will be of value to new investigators in this field and also will provide established investigators with readily accessible and in-depth methodologies for their laboratories.
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