Equipment and reagents

• Single well Boyden chambers (Neuroprobe)

• Nitrocellulose filter (12 mm diameter, 5 ^.m pore, Sartorius)

• PVP-free polycarbonate filters (12 mm diameter, 5 |xm pore, Sartorius)

• 24-well plates (Falcon, Becton Dickinson)

• 3 ml centrifuge vials (Falcon, Becton Dickinson)

• Cotton fiocs (Johnson and Johnson)

• Humidified 5% C02 incubator

• Gamma counter windowed for MCr

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