Equipment and reagents

• Tissue culture plates. 96-well, U-bottom, polystyrene (Corning-Costar)

• Assorted sterile piastic pipettes, conical tubes, tissue culture dishes, and other labware (see Protocol 1)

• Gamma counter

• Centrifuge

• Standard microscope

• Haemocyto meter

• Device for vacuum aspiration

" Repeating dispenser

• Sodium chromate s1Cr-labelled, specific activity 200-500 mCi/mg (New England Nuclear Corp.)

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