Equipment and reagents

• MICROTEST tissue culture plates. 96-well, flat-bottom, polystyrene (Falcon Plastics, Becton Dickinson Labware)

• Device for vacuum aspiration

• Repeating dispenser

• Shaking platform

• Complete medium: RPMI1540 (see Protocol 6)

• Centrifuge

• Distilled water

• Sabouraud dextrose agar (Biolife)

• C. albicans organisms

• Monocyte/macrophage effector cells, appropriately purified (see Chapters 1 and 2)

• Macrophage activators (see Protocol 1)

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