Equipment and reagents

• MICROTEST tissue culture plates. 96-well, flat-bottom, polystyrene (Falcon Plastics, Becton Dickinson Labware)

• Assorted sterile plastic pipettes, conical tubes, tissue culture dishes, and other labware (see Protocol 1)

• Gamma counter

• Equipment as in Protocol 2

• [Methyl-3H(thymidine (|3H]TdR), specific activity 2 Ci/mmol (Amersham Life Science); or ¡12SI]iododeoxyuridine (!usI]dUrd), specific activity 2000 Ci/mmol (New England Nuclear Corp.)

• OptiPhase 'HiSafe' three liquid scintillation cocktail (Wallac, EG&G Comp.)

• Ca3+-, Mg5 '-free Dulbecco's modified PBS (see Protocol 1)

(Myelone Laboratories) • Macrophage effector cells, appropriately

• Appropriate target cells; e.g. the human melanoma cell line A375 or the murine fibrosarcoma and adenocarcinoma cell lines L929, mfCSA TU5 (TU5), AMC 60, and MCA 26

purified (see Chapters 1 and 2)

• Macrophage activators (see Protocol 1)

Was this article helpful?

0 0

Post a comment