Equipment and reagents

• Heat-resistant float for holding microcentrifuge tubes

• 1.5 ml microcentrifuge tubes

• Commercial Sephadex G50 Nick column for oligonucleotide purification (Pharmacia)

• Horizontal electrophoresis apparatus appropriate for agarose gels

• Microcentrifuge

• Liquid scintillation counter

• Commercially prepared synthetic oligonucleotide primers specific for the upper and lower strands of the DNA region of interest (life Technologies or other suitable manufacturer)

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