Equipment and reagents

• Nitrocellulose membrane (Schleicher and Schuell)

• Commercial slot blot apparatus (Bio-Rad)

• Apparatus for UV cross-linking of membranes (Stratagene)

• [32P|UTP (3000 Ci/mM specific activity, Dupont/NEN): 1 mCi is sufficient for up to ten samples

• Liquid scintillation counting cocktail and glass scintillation vials of choice

• Liquid scintillation counter (Packard Instruments)

• Pasteur pipettes, sterilized by autoclaving

• Sterile microcentrifuge tubes (0.5 ml and 1.5 ml)

• Radiographic imaging film (Kodak)

• Cassettes for autoradiography (Fisher Scientific)

• Isolated nuclei from macrophage population of choice (see Protocol 8)

• Bacterial plasmid containing the gene insert of interest, and the appropriate control DNA (see Protocol 4)

• RNase-free DNase at 10 U/jxl (Boehringer Mannheim)

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