questions regarding phenotypic expression of markers, functions, and cell activation. There is considerable progress in this field with the development of new fluorescent molecules that do not photobleach as quickly as their older counterparts. Green fluorescent protein (GFP) and improved probes can be detected directly in living systems without the use of secondary reagents and new methods are becoming available to study intracellular Ca2+ compartmentation and DNA transcription in situ. These should be readily applicable to the analysis of macrophages and related cells.
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