Assessment of macrophagemediated extracellular Candida killing using a colorimetric assay

Equipment and reagents

• Microplate reader

• MICROTEST tissue culture plates, 96-well, flat-bottom, polystyrene (Falcon Plastics, Becton Dickinson Labware)

• Device for vacuum aspiration

• Repeating dispenser

• Centrifuge

• Complete medium: RPMI1640 (see Protocol 6)

• C. albicans organisms

• Monocyte/macrophage effector cells, appropriately purified (see Chapters 1 and 2)

• Distilled water

phenyltetrazolium) (Sigma Chemical) • Isopropyl alcohol (Fluka)

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