Use of Protein A in IEM

Protein A, obtainable as a purified product, is a Staphylococcus aureus bacterial cell wall protein that has the property of binding specifically to the Fc portion of IgG molecules (2) and can be used for precoating the grids prior to the ISEM procedure (7-9). This has the effect of increasing the amount of virus trapped to the grid by a modest amount but is useful when using antisera of low titre and can be used to detect distant serological relationships that would not be detected by normal ISEM (2).

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