Stabilize Hydrazide Aldehyde Bonds Optional

1. Prepare a 100 ml solution of sodium cyanoborohydride in reaction buffer (pH 6.0).

2. Add 1/20 vol of this stock drop-wise to the antibody while stirring.

3. Incubate the reaction mixture for 120 min at 4°C. Sodium cyanoborohydride is extremely toxic and should only be handled in a fume hood while wearing appropriate personal protective equipment. Cyanoborohydride-containing solutions also contain cyanide and must be disposed of according to appropriate local regulations.

4. Desalt the antibody conjugate by dialysis or gel filtration.

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