Sdspage and IEF Gels

In the case of a 1D gel (IEF or SDS-polyacrylamide gel electrophoresis [PAGE]), staining of total protein pattern can be done on one lane of the gel in which the sample or MW standard or pi standard have been loaded. Non-reversible stains more sensitive than Ponceau Red can be used (see Note 10).

1. Cut the nitrocellulose lane to be stained and immerse it in Coomassie blue solution (Subheading 2.2., step 6) for 3 min.

2. Destain for 30 min in destaining solution (Subheading 2.2., step 7) and air dry.

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