Preparation of Fragment Antigen Binding Fab and Fab2 Fragments

1.

1 M Sodium citrate, pH 3.2.

2.

Pepsin.

3.

5 M Tris-HCl.

4.

1 M Sodium acetate, pH 5.0.

5.

1 M Cysteine (see Note 6).

6.

0.05 M Ethylene diamine tetraacetic acid (EDTA).

7.

Papain.

8.

Iodoacetamide.

9.

Matrices for gel filtration (e.g., Sephacryl 400HR, Sepharose 4B, or

Superdex 200).

10.

0.05 M Sodium phosphate, 0.15 M NaCl, pH 7.4.

0 0

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