For each reaction (see Note 4) required, add the appropriate multiples of the following into a master mix (see Note 5):
Ingredient Volume per sample
5X First-strand buffer 6 |L
Dispense 30 |L of this master mix into each tube. Incubate plates (in a PCR machine) at 65°C for 5 min, then 24°C for 30 min to allow primer annealing.
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