The following method will allow researchers to develop the use of density gradient cell separations using "spiked" buffy coat preparations. Final recovery of carcinoma cells from the buffy coats can be compared with initial known percentages added to samples, allowing refinements of the technique before use with actual samples.
The described methods are divided into following subheadings: 3.1. Cell Harvesting and Cell Suspension Production, 3.2. Density Gradient Separation, and 3.3. Cell Detection Through Immunocy-tochemistry.
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