Materials

1. Gold chloride crystals.

2. 1% Aqueous trisodium citrate dihydrate.

3. 25 mM and 0.2 M Potassium carbonate.

4. 0.1 M Hydrochloric acid.

5. 10% Aqueous sodium chloride.

6. 1% Aqueous tannic acid.

7. The protein to be complexed with the gold: it is essential that the isoelectric point of the protein is known.

8. Ultracentrifuge and 10-mL ultracentrifuge tubes.

9. All distilled water to be used should be double distilled and filtered through a 0.45-pm Millipore filter.

10. All glassware should be thoroughly cleaned and siliconized. If these precautions are not taken, the gold spheres will adhere to the side vessel walls, which is evidenced by a red coloration of the glass.

11. Electron microscope facilities with Formvar/carbon-coated grids. For complete quality control, access to a monochrome image analyser is also required.

12. 1% Aqueous polyethylene glycol (Carbowax 20).

13. Phosphate-buffered saline (PBS): 0.1 M phosphate, 0.15 M sodium chloride, pH 7.4.

14. PBS containing 0.2 mg/mL of polyethylene glycol.

15. Sodium azide (Caution: highly toxic).

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