Materials

1. Infected material.

2. Electron microscope grids of 400 mesh or higher (i.e., 40 |m or less) previously coated with carbon.

3. Either fine watchmakers' forceps fitted with a suitable sized "o" ring enabling the forceps to be held closed when holding an electron microscope grid or reverse action forceps.

4. Glass slides.

5. Pestle and mortar.

6. Pasteur pipets and bulbs.

7. Glass rod with flattened end.

8. 2% Uranyl acetate in distilled water.

9. Filter paper (cut into 2.5-cm squares).

10. Grid box for storage.

11. Petri dish lined with Parafilm.

12. Wax pencil for labeling the Petri dish.

13. Distilled water.

14. Variable volume pipets to cover ranges: 0.5-10 |L, 5-50 |L, 50-200 |L, 200-1000 |L, and 1-5 mL.

15. Pipet tips of appropriate size.

16. Antisera (appropriate to the virus/es required).

17. Small beaker for washings.

18. Small microfuge tubes, sizes 0.5 and 1.5 mL.

19. Small hand grinder.

20. Grinding bags.

21. Buffer, for example, 0.06 Mphosphate buffer, pH 6.5 (for others, see relevant methods).

22. Disposable gloves.

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