There are strict regulations governing the welfare of laboratory animals used for antibody production in most countries. Before deciding on a particular approach for antibody production it is important that the appropriate authorities are contacted with a project proposal to ensure that the methods to be used are permissible. Local ethical review committees may also have input into project design to ensure that numbers of animals used are appropriate and that other diagnostic alternatives have been investigated. Legislation and ethical review typically covers animal species, numbers to be used, immunization route, bleeding regimes, and welfare issues, such as project duration.

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