Immunizing Rabbits for Polyclonal Antiserum Production

1. Mix 0.5 mL of 1 mg/mL solution of antigen with 0.5 mL of appropriate adjuvant.

2. Inject into muscle of hind leg or subcutaneously into neck scruff.

4. Test bleed (1-2 mL) on d 54 and assess for antibody activity (i.e., ELISA).

5. Bleed from marginal ear vein on d 60 and then every 28 d until antibody titre drops.

6. Give boost dose and either commence bleeding regime 10 d later or perform terminal exsanguination under anesthesia.

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