3.1.1. Immunize Rabbits as Follows (see Note 6)

1. Subcutaneously with 50-200 ^g of immunogen in FCA at multiple sites.

2. After 2-3 wk, immunize again subcutaneously with 50-100 ^g of immunogen in FIA.

3. Repeat step 2 at 2-wk intervals. Immunizing more frequently than twice weekly is not necessary and may even be detrimental. Intervals of longer than 2 wk are fine. Bleed 10 d after immunization. Test bleed (from the ear marginal vein) after the third and fifth im munizations, then completely ex-sanguinate after a further one to four immunizations as appropriate depending upon the efficacy of the antiserum.

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