Frozen Sections and Cytocentrifuge Preparations

Cryostat sections and cytocentrifuge preparations should be air-dried for at least 1 h but preferably overnight before immuno-staining. Before immunolabeling, cryostat sections should be fixed in cold acetone for 10 min and cytospin slides should be fixed for 90 s in a 1:1 mixture of acetone and methanol at room temperature. After fixation, follow the IGSS method for paraffin sections from step 3. Cytospin preparations are usually adequately covered by standard

Pancreatic Islet Imgages
Fig. 2. Photomicrograph of P-cells in a pancreatic islet of Langerhans-IGSS labeling of insulin (magnification x400).

25-^L aliquots of reagent. If immunolabeling is weak, the sections may be permeabilized for 5 min in 0.1% trypsin in TBS containing 0.1% calcium chloride at 37°C (16).

Was this article helpful?

0 0
Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

Get My Free Ebook

Post a comment