This step can now be conducted commercially at very low cost by many companies using automated sequencers. DNA can be sent at ambient temperature by overnight mail and sequence results are returned by e-mail. The primers used depend on the vector (fUSE5 or f88-4) and those recommended by G. P. Smith copy the coding strand in reverse; f88-4/15mer, 5'-AGTAGCAGAAGCCTGAAGA; fUSE5, 5'-TGAATTTTCTGTATGAGG. The sequence thus obtained is the reverse complementary strand which must be translated back to the coding strand to get the peptide sequence.
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