DNA Isolation

1. 25X TBE buffer for agarose gel electrophoresis: Trizma base, 27 g; boric acid, 13.75 g; ethylene diamine tetraacetic acid, 2.33 g. Make up to 100 mL.

2. 2X Agarose gel sample buffer: 2XTBE; 10% sucrose or glycerol; 0.5% Bromophenol blue

3. Agarose (molecular biology grade), ethidium bromide (10 mg/mL in water), DNA size markers (Type VII, Roche Diagnostics, Lewes, UK), and standard equipment for agarose gel electrophoresis (e.g., Horizon 58 apparatus; Gibco BRL, Paisley, UK).

4. UV transilluminator.

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