David Hughes

Summary

Since their introduction 60 yr ago, immunolabeling procures for use in light microscopy have become increasingly sophisticated, with greater levels of sensitivity and versatility. This chapter reviews the range of immunocytochemical procedures now available, discussing the attributes of each method that must be considered when choosing the most appropriate approach for the tissue target concerned. The immunogold silver staining technique is described in detail because the method has many advantages over rival labeling procedures, including its dual applicability at the light and electron microscope levels.

Key Words: Immunocytochemistry; immunogold; silver enhancement; immunofluorescence; immunoenzyme; avidin biotin; IGSS; PAP; APAAP; epipolarization.

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