David C Hancock and Nicola J OReilly


The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.

Key Words: Antibody; antigen; polyclonal; immunogen; immunization; peptide; ELISA.

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